ADULT RAT HIPPOCAMIPAL PROGENITOR CELLS ALTER THE EXPRESSION LEVEL OF HIPPOCAMPAL CHOLINERGIC NEUROSTIMULATING PEPTIDE PRECURSOR PROTEIN WITH DIFFERENTIATION

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Hippocampal cholinergic neurostimulating peptide (HCNP), originally purified from young rat hippocampus, has been known to promote the differentiation of septo-hippocampal cholinergic neurons. Recently, the precursor protein of HCNP (HCNP-pp) has also received attention as a multifunctional protein. HCNP-pp is thought to be important in regulating the proliferation and differentiation of neuronal cells in vitro and in vivo by modification of the MARK cascade. In the present study, we used cultured adult rat hippocampal progenitor cells (AHPs), which are thought to be important for memory formation, and forcused on the role of HCNP-pp in adult neurogenesis. We found that HCNP-pp was intensely expressed in these cells in vitro cell culture and that HCNP-pp expression changed depending on cell lineage by immunocytochemical analysis. The expression level of HCNP-pp was positively correlated with that of microtubule-associated protein 2ab (MAP2ab) as a neuron marker (Pearson's correlation test; r=0.60, p<0.01), but not with that of receptor interacting protein (RIP) as an oligodendrocyte marker. Conversely, tha rate of reduction in the fluorescence intensity of HCNP-pp with differentitation was correlated with the rate of the increase in glial fibrillary acidic protein (GFAP) as an astrocyte marker (Pearson's correlation test; r=-0.69, p<0.01). Our results show the expression level of HCNP-pp involves, directly or indirectly, in the differentiation of cultured AHPs into some lineages.
2010-05-06

ADULT RAT HIPPOCAMIPAL PROGENITOR CELLS ALTER THE EXPRESSION LEVEL OF HIPPOCAMPAL CHOLINERGIC NEUROSTIMULATING PEPTIDE PRECURSOR PROTEIN WITH DIFFERENTIATION

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