Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure(METHODS)
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概要
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Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS)4B, an integral membrane protein.
著者
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Tanaka Torahiko
Department of Advanced Medical Science, Nihon University School of Medicine
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Hasegawa Yuichiro
Department of Obstetrics and Gynecology, Nihon University School of Medicine
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Saito Makoto
Department of Obstetrics and Gynecology, Nihon University School of Medicine
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Ikeda Masanori
Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmac
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Kato Nobuyuki
Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmac
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Kato N
Department Of Tumor Virology Okayama University Graduate School Of Medicine Dentistry And Pharmaceut
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Kato Nobuyuki
Department Of Molecular Biology Graduate School Of Medicine And Dentistry Okayama University
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Ikeda M
Department Of Tumor Virology Okayama University Graduate School Of Medicine Dentistry And Pharmaceut
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Kato Nobuyuki
Tumor Virology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences
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Numata Kazushi
横浜市立大学附属市民総合医療センター消化器病センター
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Tanaka Torahiko
Department Of Advanced Medical Science Nihon University School Of Medicine:(present Office)division
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Hasegawa Yuichiro
Department Of Materials And Applied Chemistry College Of Science And Technology Nihon University
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Hasegawa Yuichiro
Department Of Obstetrics And Gynecology Nihon University School Of Medicine:open Research Center For
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Saito Makoto
Department Of Obstetrics And Gynecology Nihon University School Of Medicine:open Research Center For
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Ikeda Masanori
Department Of Tumor Virology Okayama University Graduate School Of Medicine Dentistry And Pharmaceut
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Kato Nobuyuki
Department Of Materials Science And Engineering School Of Science And Engineering Waseda University
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Ikeda Masanori
Department Of Electronics College Of Science And Technology Nihon University
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Kato Nobuyuki
Department Of Tumor Virology Okayama University Graduate School Of Medicine Dentistry And Pharmaceut
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Ikeda Masanori
Department Of Electrical And Electronic Engineering College Of Engineering Nihon University
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Saito Makoto
Department Of Chemistry College Of General Education Tohoku University
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Ikeda Masanori
Department Of Tumor Virology Okayama University Graduate School Of Medicine Dentistry And Pharmaceut
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Saito Makoto
Department Of Bacteriology Aichi Prefectural Institute Of Public Health
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Kato Nobuyuki
Department Of Tumor Virology Okayama University Graduate School Of Medicine Dentistry And Pharmaceut
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Kato Nobuyuki
Department of Applied Chemistry, Faculty of Engineering, Osaka University
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