SYNTHESIS AND CELLULAR UPTAKE OF FLUORESCEIN INTERCALATED LAYERED DOUBLE HYDROXIDE(2010 TMC Special Paper)

概要

論文の詳細を見る
Layered double hydroxide (LDH) is widely known as anionic clay and has an anion exchange property. The cellular uptake of LDH has received considerable attention in recent years in view of their potential technological importance as materials for drug delivery due to low cytotoxicity, good biocompatibility and controlled release of drug. To investigate the cellular uptake behaviour of LDH, the stable and labeled highly fluorescent might be required. In this paper, we focused on fluorescein disodium salt (Fluo), as a guest in fluorescence dye, the intercalation of Fluo into Mg-Al LDH by coprecipitation method has been investigated. To define the abilities of LDH as materials for drug delivery, the cellular uptake of the Fluo/LDH for the mammalian cells, L929 and HeLa, has also been studied. The characterization of the Fluo/LDH was carried out by using XRD, FT-IR and element chemical analysis. The Fluo/LDH had a layered structure, which the two kinds of basal spacing (003) was 1.58nm and 0.77nm. These values indicated that the interlayer place of Fluo anion changed for horizontal orientation to vertical orientation with increasing Fluo content. According to the fluorescence, confocal and TEM images of cells, the fluorescence of cellular cytoplasm and the internalized Fluo/LDH particles were observed. We demonstrated that the Fluo/LDH was internalized into the mammalian cells by the cellular endocytosis.