4. ナガガジ由来III型不凍蛋白質の細胞保護機能に関する研究(平成20年度第54回低温生物工学会年会)

概要

論文の詳細を見る
Antifreeze protein from Notched-fin eelpout (nfeAFP) has an ability to preserve mammalian cells in hypothermic condition. We compared the preservation ability between the naturally extracted isoform mixtures (denoted nfeAFPs) and a recombinant single isoform (denoted nfeAFP6) through observation of cultivated cells (HepG2) preserved at 4℃ for 24 hr with Euro-Collins solution in the presence of 0 - 10 mg/ml of the AFP samples. The preserved cells were examined by fluorescence microscopy after staining with calcein-AM and propidium iodide and by evaluations of extracellular lactate dehydrogenase (LDH) and intracellular ATP content. It appeared that nfeAFPs preserve the cell viability more effectively than nfeAFP6. In addition, the preservation ability increased dose-dependently and is maximized at 10 mg/ml of the protein concentration. These results suggest that addition of nfeAFP decreases hypothermic stress and the mixture of nfeAFP isoforms is more effective than the single isoform.
2008-12-30