3. 不凍蛋白質の活性に対する可溶性合成高分子の効果(平成20年度第54回低温生物工学会年会)

概要

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Antifreeze protein (AFP) causes thermal hysteresis (TH) through its inhibitory action of the ice crystal growth, for which preservation effect is expected for a water-containing gel in a frozen state. A very weak TH activity was also reported for poly-vinyl alcohol (PVA). Here we performed TH measurements for type III AFP from Notched-fin eelpout (Zoarces elongates Kner) at various AFP concentrations in the absence and presence of PVA, and found that approximately 1.8-fold enhancement of TH is achieved by addition of 50mg/ml of PVA. We further examined structure of 0.5% agarose gel before and after freezing at -18℃ under various concentrations of AFP, PVA, and their mixture. It appeared that the gel structure was completely destroyed after freezing without addition of AFP or PVA, while it was preserved in the presence of 0.09mg/ml AFP or 50mg/ml PVA. Structure of the frozen gel was also preserved by a mixture of 0.03mg/ml AFP and 25mg/ml PVA. These results suggest that AFP provides a freeze-tolerance to water-containing gels, for which a required amount of AFP can be saved by addition of PVA.
2008-12-30