cDNA Cloning and Analysis of the Chicken Homolog (chApex1)of APEX Nuclease, a Multifunctional DNA Repair Enzyme
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概要
- 論文の詳細を見る
We have cloned and analyzed the cDNA (chApex1) for the chicken homolog of APEX nuclease, a multifunctional DNA repair enzyme. Chicken Apex cDNA fragments were first amplified from Chick Embryo Lambda cDNA Library by nested PCR (polymerase chain reaction) using primers constructedon the basis of information of highly conserved regions of the amino acid sequences among mouse, rat and human APEX nuclease. Almost full-size chicken Apex cDNA was cloned from the Chick Embryo Lambda cDNA Library using the amplified chicken Apex cDNA fragments as a probe.The cloned cDNA was 1751 nucleotide long encoding a protein consisting of 300 amino acids with a predicted molecular mass of 33 kDa. The amino acid sequence exhibits a high homology with the sequence of the mammalian APEX nuclease (64%, 62% and 63% identities/300 amino acids with those of the human, mouse and rat APEX nuclease, respectively). The homologies of the amino acid sequences between chicken and mammalian APEX nucleases occurred along nearly the entire length of the sequences.
- 中国学園大学の論文
著者
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Nakamura Takashi
Department of Cardiology, Saiseikai Shigaken Hospital
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Seki Shuji
Departments Of Emergency Medicine And Surgery Okayama University Okayama National Hospital And Kagaw
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Seki Shuji
Department Of Human Nutrition Faculty Of Contemporary Life Science Chugokugakuen University
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Seki Shuji
Department Of Biochemistry Cancer Institute Okayama University Medical School
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IKEDA Shogo
Department of Biochemistry, Cancer Institute, Okayama University Medical School
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Ikeda Shogo
Department Of Biological Chemistry Okayama University Of Science
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Ikeda Shogo
Department Of Biochemistry Cancer Institute Okayama University Medical School
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Sarker Altaf
Department Of Cellular And Molecular Biology Life Science Division Lawrence Berkeley National Labora
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Seki Yuichi
Tsuyama Medical Examination Center of Chugoku Occupational Health Association
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Nakamura Takashi
Department Of Molecular Biology Okayama University Graduate School Of Medicine And Dentistry
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Nakamura Takashi
Department Of Cardiology Saiseikai Shiga Hospital
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SarkerAltaf H.
Department of Cellular and Molecular Biology, Life Science Division, Lawrence Berkeley National Laboratory, University of California
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