Genomic structure and sequence of a chicken homolog(chNthl1) of Escherichia coli endonuclease III with those ofthe adjacent parts of chTsc2 and chSlc9a3r2 genes

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概要

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• Genomic cloning and sequencing of a chicken homolog (chNthll) for Escherichia coli endonuclease Ⅲ, that is involved in pyrimidine base excision repair, were performed. The sequence covered the entire chNthll gene consisting of six exons and five introns spanning 4.9kb with 5' flanking (6.6kb) and 3' flanking (3.lkb) regions. The boundaries between exon and intron follow the GT/AG rule. The sequenced 5' flanking region lacks typical TATA and CAAT boxes, but contains a CpG island having putative binding sites for several transcription factors such as Ests 1 and Spl. The chNthll gene lies immediately adjacent to the tuberous sclerosis 2 (chTsc2) gene in a 5'-to-5' orientation. Downstream of the chNthll gene, the gene for the regulatory factor 2 (chSlc9a3r2) of the solute carrierfamily 9 (sodium/hydrogen exchanger), isoform A3, lies in a 3'-to-3' orientation. The genomic organization of the chNthll gene and the relative orientation of the gene with the adjacent genes of chTsc2 and chSlc9a3r2 are similar to those in human and mouse genomes.

• 中国学園大学の論文

著者

• Nakamura Takashi

  Department of Cardiology, Saiseikai Shigaken Hospital

• Seki Shuji

  Departments Of Emergency Medicine And Surgery Okayama University Okayama National Hospital And Kagaw

• Seki Shuji

  Department Of Human Nutrition Faculty Of Contemporary Life Science Chugokugakuen University

• Seki Shuji

  Department Of Biochemistry Cancer Institute Okayama University Medical School

• IKEDA Shogo

  Department of Biochemistry, Cancer Institute, Okayama University Medical School

• Ikeda Shogo

  Department Of Biological Chemistry Okayama University Of Science

• Ikeda Shogo

  Department Of Biochemistry Cancer Institute Okayama University Medical School

• Sarker Altaf

  Department Of Cellular And Molecular Biology Life Science Division Lawrence Berkeley National Labora

• Seki Yuichi

  Tsuyama Medical Examination Center of Chugoku Occupational Health Association

• Nakamura Takashi

  Department Of Molecular Biology Okayama University Graduate School Of Medicine And Dentistry

• Nakamura Takashi

  Department Of Cardiology Saiseikai Shiga Hospital

• SarkeAltaf H.

  Department of Cellular and Molecular Biology, Life Science Division, Lawrence Berkeley National Laboratory, University of California

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