Ribosomal RNA gene (rDNA) distribution in human leukemia cells by in situ hybridization on chromosome.

概要

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By in situ hybridization on chromosome, phytohemagglutinin (PHA)-stimulated lymphocytes obtained from normal individuals showed slight polymorphism in terms of distribution of rDNA among Nucleolar Organizer Region (NOR) chromosomes probably due to racial differences, although their interindividual distinct polymorphism had been reported in the U.S.A. Three chronic and one acute myelogenous leukemias (CML and AML) and one chronic monocytic leukemia (CMoL) were also analysed for the distribution of rDNA among NORs. The distribution patterns in leukemia cells were found to be significantly different from those in the cells of normal individuals. Although genetic alteration of normal leukocytes was not disregarded, the changes of rDNA distribution in leukemia cells are demonstrated in this study. The Ph1 chromosome in CML carried a greater amount of rDNA. The rDNA distribution in Ph1-negative cells obtained from patients showed almost the same pattern as that of Ph1-positive cells. In AML, the t(8;21) carried a smaller amount of rDNA. Trisomic chromosome 21 in CMoL carried extra rDNA copies on its NOR. Based on these data, leukemia cells seem to show variability of rDNA distribution especially on marker chromosomes, contrary to the non-polymorphic patterns of normal lymphocytes. Thus a strong relationship between marker formation and abnormal distribution of rDNA could be suggested.
神戸大学の論文