実験白血病の前白血病期における白血病細胞増殖の病理組織学的、細胞遺伝学的研究

概要

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It is unknown how malignant cells start to grow in the body. Proliferation of leukemia cells induced by leukemogenic compounds such as 7, 12-dimethylbenz [a] anthracene (DMBA) was studied by serial biopsies of the liver and chromosome analysis of bone marrow cells in the Long-Evans rat. The following results were obtained. 1) To induce leukemia, several pulse doses of DMBA were necessary. The incidence became higher as the doses of DMBA increased and leukemia cells started to grow after the last treatment. 2) Atypical cells of leukemic nature frequently appeared in the sinusoids of the liver just after the last DMBA treatment. In some animals, this lead to irreversible growth of leukemic cells and in others the growth was inhibited. In the latter cases, atypical cells were found destroyed within microgranulomatous foci in later biopsies. 3) On the other hand, cells with aneuploidy or marker chromosomes were found in the bone marrow of the rats with leukemogens such as DMBA and 1-nitroso-1-butylurea(NBU) as early as 1 week after the first treatment. The overal incidence of abnormal cells was 0.04% in DMBA group and 1.5% in NBU group. Two early leukemias had mosaic growth of clonal leukemia cells.
神戸大学の論文