The Validity of Marker-assisted Selection Using DNA Markers Linked to a Pear Scab Resistance Gene (Vnk) in Two Populations

概要

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Marker-assisted selection (MAS) using 5DNA markers linked to the pear scab resistance derived from 'Kinchaku' (Vnk) was examined in a pear breeding program. The relationship between DNA markers and scab resistance was investigated. All individuals without both DNA markers of STS-OPW2 and STS-OPO9 derived from 'Kinchaku' showed scab symptoms with heavy sporulation on leaves. Individuals possessing these markers exhibited no symptoms, or necrosis with or without weak sporulation on petioles which was localized and did not affect growth, and were classified as resistant. Hence, elimination of individuals that have neither STS-OPW2 nor STS-OPO9 would be a suitable strategy to select for scab resistance in Japanese pear breeding when introgressing Vnk from 'Kinchaku'.
園芸学会の論文