Neuroprotective Effect of Sanguisorbae Radix against Oxidative Stress-Induced Brain Damage : in Vitro and in Vivo(Pharmacology)

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概要

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• Sanguisorbae radix(SR), the root of Sanguisorba officinalis L.(Rosaceae), has been traditionally used for its anti-inflammatory, anti-infectious and analgesic activities in Korea. Previous work has shown that SR prevents neuronal cell damage induced by Aβ (25-35) in cultured rat cortical neurons. The present study was carried out to further investigate the neuroprotective effect of SR on oxidative stress-induced toxicity in primary culture of rat cortical neurons, and on ischemia-induced brain damage in rats. SR, over a concentration range of 10-50μg/ml, inhibited H_2O_2 (100μM)-induced neuronal death, which was significantly inhibited by MK-801 (5μM), an N-methyl-D-aspartate (NMDA) receptor antagonist, and verapamil (20μM), an L-type Ca^<2+> channel blocker. Pretreatment of SR (10-50μg/ml), MK-801 (5μM), and verapamil (20μM) inhibited H_2O_2-induced elevation of intracellular Ca^<2+> concentration ([Ca^<2+>]_i) measured by a fluorescent dye., Fluo-4 AM. SR (10-50μg/ml) inhibited H_2O_2-induced glutamate release into medium measured by HPLC, and generation of reactive oxygen species (ROS) measured by 2',7'-dichlorodihydrofluorescein diacetate (H_2DCFDA). In vivo, SR prevented cerebral ischemic injury induced by 2-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct and edema were significantly reduced in rats that received SR (10,30mg/kg, orally), with a corresponding improvement in neurological function. Catechin isolated from SR inhibited H_2O_2-induced neuronal death in cultures. Taken together, these results suggest that SR inhibits H_2O_2-induced neuronal death by interfering with the increase of [Ca^<2+>]_i, and inhibiting glutamate release and generation of ROS, and that the neuroprotective effect of SR against focal cerebral ischemic injury is due to its anti-oxidative effects. Thus SR might have therapeutic roles in neurodegenerative diseases such as stroke.

• 社団法人日本薬学会の論文
• 2008-11-01

著者

• Hguyen Thi

  College Of Veterinary Medicine Chungbuk National University

• Nguyen Thi

  College Of Technology Vietnam National University Hanoi

• BAN Ju

  Department of Pharmacology, College of Medicine, Kyung Hee University

• KIM Ju

  College of Veterinary Medicine, Chungbuk National University

• JU Hyun

  College of Veterinary Medicine, Chungbuk National University

• KOH Sang

  Safety Assessment Center, Korea Testing and Research Institute for Chemical Industry

• SONG Kyung-Sik

  College of Agriculture and Life-Science, Kyungpook National University

• SEONG Yeon

  College of Veterinary Medicine, Chungbuk National University

• CHO Soon

  College of Veterinary Medicine, Chungbuk National University

• Nguyen Thi

  College Of Veterinary Medicine Chungbuk National University

• Song Kyung-sik

  College Of Agriculture And Life-sciences Kyungpook National University

• Song Kyung-sik

  韓国

• Kim Ju

  College Of Veterinary Medicine Chungbuk National University

• Cho Soon

  College Of Veterinary Medicine Chungbuk National University

• Seong Yeon

  Coll. Of Veterinary Medicine Chungbuk National Univ. Kor

• Ban Ju

  School Of Dentistry Dankook University

• Ju Hyun

  College Of Veterinary Medicine Chungbuk National University

• Seong Y

  College Of Agriculture And Life Science Kyungpook National University

• Seong Yeon

  College Of Agriculture And Life Science Kyungpook National University

• Koh Sang

  Safety Assessment Center Korea Testing And Research Institute For Chemical Industry

• Kim Ju

  College Of Pharmacy Yeungnam University

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