P-53 コムラサキシメジ(Lepista sordida)と芝の共生に関する化学的解明(ポスター発表の部)

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During the spring or summer a circle or are of stimulated grass or of toadstools may appear in lawns and other grassy places. These are turf abnormalitines called fairy rings, "Fairy ring diseases" are caused by about sixty kinds of mushroom-forming fungi. Among fairy-ring forming mushrooms, Lepista sordida was investigated as a source of plant-growth regulators The culture broths of L. sordida were filtered The filtrates were partitioned between hexane and water, then ethyl acetate and water. The aqueous layer was dried under reduced pressure, and the resulting residue was extracted with ethanol. Effect of the obtamed fractions on turf growth was examined. The bioassay-guided separation of both ethyl acetate soluble part and ethanol soluble part gave compounds 1 (2-azahypoxanthine) and 2 (1H-umdazole-4-carboxamide), respectively Root growth of bentgrass seedlings exposed to 10μM of 1 was accelerated(320% of control) Compound 2 also promoted the root elongation(134% of control). Shoot and root of bentgrass (Agrostis stolomfera) were successively extracted with hexane, ethyl acetate, ethanol, and then water Since the hexane soluble part of the root showed significant inhibition against growth of L. sordida, it was repeatedly chromatographed on the basis of the result of the bioassay. As a result, compound 3 was purified This compound inhibited the growth of the fungus The symbiotic relation between L sordida and turfgrass is now being elucidated.
天然有機化合物討論会の論文
2007-08-24

P-53 コムラサキシメジ(Lepista sordida)と芝の共生に関する化学的解明(ポスター発表の部)

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