P-53 新規ミオシン軽鎖キナーゼ阻害物質MS-681類の構造(ポスター発表の部)

概要

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MS-681s (1〜4) are potent inhibitors of myosin light chain kinase, elaborated by Myrothecium species. Molecular formulae of 1〜4 were determined by high resolution FABMS and NMR data. Planar structure of 2 was established by amino acid analysis of the acid hydrolysate, 2D NMR analyses and FAB MS/MS experiments. The structures of 1, 3 and 4, which differ from 2 only in amino acid residues, were elucidated by comparison of the spectral data with those of 2. Minor components were recognized in crude samples of 1〜4, and FAB MS/MS analyses proved them as spermidine moiety derivatives. Stereochemistry of their constituting amino acids was determined by chiral GC analyses, and the absolute configuration of the polyamine moiety was determined to be S by comparison of 8, which was derived from each 1〜4, with synthetic authentic samples. MS-681s are novel octapeptides consisting of a N-protected terminal amino acid, four unusual amino acid residues (α-aminoisobutyric acid and DL-isovaline) and a polyamine bonded to C-terminal amino acid.
天然有機化合物討論会の論文
1994-09-20