Alteromonas thalassomethanolica YK4042を用いた食品,医薬品中のビタミンB_<12>定量法

概要

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Microbiological assay of vitamin B_<12> in several foods and drugs by use of a marine methanol-utilizing bacterium, Alteromonas thalassomethanolica YK4042 is described. A. thalassomethanolica required cyanocobalamin or some of its analogues for growth. The relative activities of cyanocobalamin, adenosylcobalamin, hydroxocobalamin, methylcobalamin, cobinamide dicyanide for the growth were 1:1:0.9:0.9:1.3. Application of Sep-pak C_<18> cartridge for the elimination of cobinamide dicyanide was proved to be effective. Freeze-dried A. thalassomethanolica cells were stable for at least 16 months at -20℃ their direct use in a microbiological assay reduced day-to-day variation in the inoculum. Samples of 37 foods and foodstuffs, and 4 drugs were analyzed for content of vitamin B_<12> as cyanocobalamin by A. thalassomethanolica and Lactobacillus leichmannii. There was good agreement in B_<12> values between these two test organisms.
日本ビタミン学会の論文
1989-02-25