16S rRNA Sequence-based Rapid and Sensitive Detection of Aquatic Bacteria by On-chip Hybridization Following Multiplex PCR

スポンサーリンク

概要

• 論文の詳細を見る

• We applied transcription in vitro following multiplex PCR in order to improve the sensitivity and rapidity of microbial detection in aquatic environment with an oligonucleotide microarray. Transcripts of 16S rRNA gene were fluorescently labeled and hybridized on fabricated oligonucleotide chip. The assay sensitivity was evaluated by detecting cultured bacteria inoculated into natural river water. By using our procedure, the assay was completed more rapidly (6 hr) than conventional oligonucleotide microarray assays (>12 hr), and its sensitivity was improved: detection limit was decreased by one order of magnitude. This method might be useful for monitoring pathogenic bacteria in aquatic environments.

• 公益社団法人日本薬学会の論文
• 2008-02-01

著者

• YAMAGUCHI NOBUYASU

  Graduate School of Pharmaceutical Sciences, Osaka University

• NASU MASAO

  Graduate School of Pharmaceutical Sciences, Osaka University

• Nasu M

  Graduate School Of Pharmaceutical Sciences Osaka University

• Nasu Masao

  Graduate School Of Pharmaceutical Sciences Osaka University

• TANI KATSUJI

  Graduate School of Pharmaceutical Sciences, Osaka University

• Yamaguchi Nobuyasu

  Graduate School Of Pharmaceutical Sciences Osaka University

• Tani Katsuji

  Faculty Of Pharmacy Osaka Ohtani University

• Ichijo Tomoaki

  Graduate School of Pharmaceutical Sciences, Osaka University

• Ichijo Tomoaki

  Graduate School Of Pharmaceutical Sciences Osaka University

関連論文

• Similarity of Bacterial Community Structure between Asian Dust and Its Sources Determined by rRNA Gene-Targeted Approaches
• Rapid Detection of Starved Escherichia coli with Respiratory Activity in Potable Water by Signal-Amplified in situ Hybridization Following Formazan Reduction
• Application of Real-Time Long and Short Polymerase Chain Reaction for Sensitive Monitoring of the Fate of Extracellular Plasmid DNA Introduced into River Waters
• Effecient Transformation of Marchantia polymorpha That is Haploid and Has Very Small Genome DNA
• In Situ Analysis of Community Structure in Activated Sludge with 2-Hydroxy-3-Naphthoic Acid-2'-Phenylanilide Phosphate and Fast Red TR In Situ Hybridization
• A Combination of Direct Viable Counting, Fluorescence in situ Hybridization, and Green Fluorescent Protein Gene Expression for Estimating Plasmid Transfer at the Single Cell Level
• Oligonucleotide Probes for Phylogenetic Detection of Waterborne Bacteria
• Stimulatory Effect of Glutamine and Pyruvate on Plasmid Transfer between Pseudomonas Strains
• Soil Microbial Community Structure in an Asian Dust Source Region (Loess Plateau)
• 1-23 Profiling of bacterial community structure on Asian dust particles by T-RFLP(Community structure 2,Oral presentation)
• Response of River Water Bacterial Communities to Aniline in Two Biodegradation Test Systems
• Bacterial Population Dynamics in a Reverse-Osmosis Water Purification System Determined by Fluorescent Staining and PCR-Denaturing Gradient Gel Electrophoresis
• Inactivation of Bacteria in Freshwater by Momentary Decompression Following High Pressurization
• PA-17 Diversity of Legionella pneumophila in aquatic environment in Japan and Southeast Asian region(Aquatic ecosystem,Poster presentation A)
• Inactivation of Bacteria in Freshwater by Momentary Decompression Following High Pressurization
• PB-19 GyrB gene expression as indicator of bacterial growth activity(Waste water treatment ecosystem,Poster presentation B)
• 1-19 High-Frequency Phage-Mediated Gene Transfer among Enterobacteriaceae. Determined at the Single-Cell Level(Gene transfer,Oral presentation)
• Rapid Identification and Enumeration of Antibiotic Resistant Bacteria in Urban Canals by Microcolony-Fluorescence in Situ Hybridization
• Rapid Monitoring of Escherichia coli in Southeast Asian Urban Canals by Fluorescent-Bacteriophage Assay
• Bacterial Community Composition and Activity in Urban Rivers in Thailand and Malaysia
• Rapid In Situ Enumeration of Physiologically Active Bacteria in River Waters using Fluorescent Probes
• A Great Leap forward in Microbial Ecology
• Occurrence of Escherichia coli O157 : H7 in river water determined by flow cytometry
• Staphylococcus epidermidis Forms Floating Micro-colonies in Platelet Concentrates at the Early Stage of Contamination
• 16S rRNA Sequence-based Rapid and Sensitive Detection of Aquatic Bacteria by On-chip Hybridization Following Multiplex PCR
• Detection of Food Poisoning Bacteria in Fresh Vegetables Using DNA Microarray
• Development of Phylogenetic Oligonucleotide Probes for Screening Foodborne Bacteria
• Change in the Bacterial Community of Natural River Biofilm during Biodegradation of Aniline-Derived Compounds Determined by Denaturing Gradient Gel Electrophoresis
• Estimation of Bacterial Biovolume and Biomass by Scanning Electron Microscopic Image Analysis
• A Great Leap forward in Microbial Ecology
• Soil Microbial Community Structure in an Asian Dust Source Region (Loess Plateau)
• Environmental disease: environmental alteration and infectious disease (Special feature on environmental change, pathogens and human linkages)
• Rapid On-chip flow Cytometric Detection of Listeria monocytogenes in Milk
• High Prevalence of qnr and aac(6’)-Ib-cr Genes in Both Water-Borne Environmental Bacteria and Clinical Isolates of Citrobacter freundii in China
• High Prevalence of qnr and aac(6')-Ib-cr Genes in Both Water-Borne Environmental Bacteria and Clinical Isolates of Citrobacter freundii in China
• Environment-KHV-carp-human linkage as a model for environmental diseases
• Environmental disease : environmental alteration and infectious disease
• Break Down of Asian Dust Particle on Wet Surface and Their Possibilities of Cause of Respiratory Health Effects
• Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
• Bacterial Monitoring with Adhesive Sheet in the International Space Station-"Kibo", the Japanese Experiment Module
• 16S Ribosomal RNA Gene-Based Phylogenetic Analysis of Abundant Bacteria in River, Canal and Potable Water in Bangkok, Thailand
• Bacterial Monitoring with Adhesive Sheet in the International Space Station-"Kibo", the Japanese Experiment Module

もっと見る 閉じる

スポンサーリンク