Estimation of Bacterial Biovolume and Biomass by Scanning Electron Microscopic Image Analysis

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概要

• 論文の詳細を見る

• A scanning electron microscopic (SEM) image analysis system was used to measure bacterial biovolume in fresh water. Halation, which is a problem encountered in epifluorescence microscopy, is avoided in SEM image analysis. The biovolume-to-carbon and nitrogen conversion factors, which are used to estimate bacterial biomass, were determined in four standard strains and five river water isolates. These factors for carbon and nitrogen were 0.34±0.06 pg C/μm^3 and 0.094±0.014 pg N/μm^3 respectively. There was no noticeable difference among bacterial species or growth phases. Bacterial size and biomass were measured in polluted and unpolluted river water. The average volume of bacterial cell was twice as high in polluted water, while the bacterial biomass was 35 times higher than in unpolluted water.

• 日本微生物生態学会の論文
• 1996-06-30

著者

• NASU MASAO

  Graduate School of Pharmaceutical Sciences, Osaka University

• Nasu M

  Graduate School Of Pharmaceutical Sciences Osaka University

• Nasu Masao

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Science Osaka University

• YAMAGUCHI NOBUYASU

  Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• Tani Katsuji

  Environmental Science And Microbiology Faculty Of Pharmacy Osaka Ohtani University:environmental Sci

• Tani Katsuji

  Faculty Of Pharmacy Osaka Ohtani University

• Chen Ji

  Environmental Science And Microbiology Faculty Of Pharmaceutical Science Osaka University

• Yamaguchi Nobuyasu

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Science Osaka University

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