Dynamics of Methanotrophs during in situ Bioremediation

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概要

• 論文の詳細を見る

• Changes in diversity of type I and type II methanotrophs during a biostimulation field experiment were investigated for 73 days after the start of treatment by denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rDNA fragments. DGGE profiles of the type I and type II methanotrophs yielded simple banding patterns. The type I methanotrophs' profile showed a single intense band at day 10 of biostimulation, and sequence analysis showed 99% similarity with Methylobacter psychrophilus. The type II methanotrophs' profile showed a single band appeared at day 31, becoming an intense band after day 40. Sequence analysis of this band pattern showed 99% similarity with Methylocystis parvus. These results suggest a possible shift of numerically dominant methanotrophs from type I to type II during the field experiment.

• 日本微生物生態学会の論文

著者

• Nasu Masao

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Science Osaka University

• Tani Katsuji

  Environmental Science And Microbiology Faculty Of Pharmacy Osaka Ohtani University:environmental Sci

• Tani Katsuji

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Sciences Osaka University

• Nasu M

  Osaka Univ. Osaka Jpn

• Iwamoto Tomotada

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Sciences Osaka University

• Fujimoto Kazuo

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Sciences Osaka University

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