Effecient Transformation of Marchantia polymorpha That is Haploid and Has Very Small Genome DNA

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概要

• 論文の詳細を見る

• The genomic DNA content of a cultured cell of Marchantia polymorpha HYA-2F was examined using a flow cytometer. It was estimated be 0.32 pg (C), with a G+C content of 57.1%. The DNA content was less than that of Arabidopsis thaliana. The frequency of transformation by Agrobacterium tumefaciens using a binary vector plasmid pBI121 in the presence of acetosyringone was aproximately 10%. GUS expression analysis and Southern blotting analysis of the genomic DNA of transformants revealed that all regions of T-DNA on plasmid pBI121 were integrated into the genome of M. polymorpha.

• 社団法人日本生物工学会の論文
• 1997-12-25

著者

• NASU MASAO

  Graduate School of Pharmaceutical Sciences, Osaka University

• Katoh Kenji

  Bioorganic Chemistry Aburabi Laboratories

• Nasu M

  Graduate School Of Pharmaceutical Sciences Osaka University

• Nasu Masao

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Sciences Osaka University

• Nasu Masao

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Science Osaka University

• TANI KATSUJI

  Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• HATTORI CHIZUKO

  Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• HONDA MOTOYASU

  Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• SHIMAOKA TAISEI

  Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• YAMAGUCHI NOBUYASU

  Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• Tani Katsuji

  Environmental Science And Microbiology Faculty Of Pharmacy Osaka Ohtani University:environmental Sci

• Honda Motoyasu

  Environmental Science And Microbiology Faculty Of Pharmaceutical Sciences Osaka University

• Tani Katsuji

  Faculty Of Pharmacy Osaka Ohtani University

• Shimaoka Taisei

  Environmental Science And Microbiology Faculty Of Pharmaceutical Sciences Osaka University

• Hattori Chizuko

  Environmental Science And Microbiology Faculty Of Pharmaceutical Sciences Osaka University

• Yamaguchi Nobuyasu

  Environmental Science And Microbiology Graduate School Of Pharmaceutical Science Osaka University

• HONDA MOTOYASU

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University

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