Stimulatory Effect of Glutamine and Pyruvate on Plasmid Transfer between Pseudomonas Strains

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概要

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• The effect of amino acids (glutamine, asparagine, and serine) and organic acids (citrate, malate, and pyruvate) on plasmid transfer was investigated on agar plates using Pseudomonas strains differing in physiological status. Counts by culture-dependent and culture-independent methods showed that the presence of organic compounds as the sole nutrient source increased the absolute number of transconjugants. Morevover, the presence of glutamine and pyruvate as the sole nutrient source resulted in a higher transfer frequency. This stimulatory effect of pyruvate was also investigated in short-term conjugation experiments under nutrient-amended conditions with glucose. Compared with glutamine, the presence of pyruvate resulted in a higher transfer frequency after 5h of conjugation with active cells. The present study confirmed the importance of organic compounds and the physiological status of bacteria to plasmid transfer, and provided evidence that certain organic compounds (glutamine and pyruvate) can stimulate the growth of transconjugants and/or conjugal gene exchange between Pseudomonas spp.

• 日本微生物生態学会の論文

著者

• YAMAGUCHI NOBUYASU

  Graduate School of Pharmaceutical Sciences, Osaka University

• NASU MASAO

  Graduate School of Pharmaceutical Sciences, Osaka University

• Nasu M

  Graduate School Of Pharmaceutical Sciences Osaka University

• Nasu Masao

  Graduate School Of Pharmaceutical Sciences Osaka University

• TANI KATSUJI

  Graduate School of Pharmaceutical Sciences, Osaka University

• Yamaguchi Nobuyasu

  Graduate School Of Pharmaceutical Sciences Osaka University

• Jorquera Milko

  Graduate School Of Pharmaceutical Sciences Osaka University:instituto De Agroindustria Universidad D

• Tani Katsuji

  Faculty Of Pharmacy Osaka Ohtani University

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