Rapid Monitoring of Escherichia coli in Southeast Asian Urban Canals by Fluorescent-Bacteriophage Assay

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概要

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• The microbial water quality in canals of metropolitan Bangkok, Thailand, was assessed using the fluorescent bacteriophage assay (FBA) and fluorescence in situ hybridization (FISH). When FISH was used with oligonucleotide probes targeted to Escherichia coli (E. coli) and anaerobic Bacteroides group, <0.1% to 7% and <0.1% to 11 %, respectively, of the total bacteria were identified. Estimates of viable E. coli determined by FBA with 4', 6-diamidino-2-phenylindole (DAPI)-labeled T4 bacteriophage, which accounted for 1 to 8% of total cells, were significantly higher than those of fecal coliforms determined by the most probable number method. The FBA procedure can specifically detect viable E. coli in canal water samples that are grossly contaminated with fecal flora within 30 min, and it can quickly deliver accurate findings for risk assessment of environmental contamination in water samples.

• 公益社団法人日本薬学会の論文
• 2006-12-01

著者

• KENZAKA TAKEHIKO

  Graduate School of Pharmaceutical Sciences, Osaka University

• NASU MASAO

  Graduate School of Pharmaceutical Sciences, Osaka University

• Nasu M

  Graduate School Of Pharmaceutical Sciences Osaka University

• Nasu Masao

  Graduate School Of Pharmaceutical Sciences Osaka University

• Kenzaka Takehiko

  Graduate School Of Pharmaceutical Sciences Osaka University

• KENZAKA Takehiko

  Faculty of Pharmaceutical Sciences,Osaka University

• Kenzaka Takehiko

  Faculty Of Environment And Resource Studies Mahidol University:graduate School Of Pharmaceutical Sci

• Utrarachkij Fuangfa

  Faculty of Public Health, Mahidol University

• Suthienkul Orasa

  Faculty of Public Health, Mahidol University

• Suthienkul Orasa

  Faculty Of Public Health Mahidol University

• Utrarachkij Fuangfa

  Faculty Of Public Health Mahidol University

• Kenzaka Takehiko

  Faculty Of Environment And Resource Studies Mahidol University:graduate School Of Pharmaceutical Sciences Osaka University

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