An Improved Method for Basic Hydrolysis of Isoflavone Malonylglucosides and Quality Evaluation of Chinese Soy Materials
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概要
- 論文の詳細を見る
Basic hydrolysis procedure is often included in the sample preparation in order to quantify malonylglucosides or acetylglucosides of soy materials. However, it is preferable not to use NaOH as a hydrolytic reagent considering the effect of its alkalinity on the successive injection to HPLC and low acidity of soy isoflavones. This paper presents an improved method for basic hydrolysis using ammonia as a hydrolytic reagent without the additional neutralization step. Moreover, by means of HPLC and LC-MS methods, a systematic quality evaluation of natural soy materials from Chinese markets were established and discussed, inclusive of soybeans, black soybeans, defatted soy flours, as well as the distribution of isoflavones in the seed coat, hypocotyl and cotyledon. The results indicate that HPLC profiling patterns of originating various isoflavone constituents of Chinese soybeans was similar to those of Japanese ones, and those of Chinese black soybeans was similar to those of American ones. The average content level of total soy isoflavones of Chinese soybeans and black soybeans were a little lower than that of American and Japanese ones. Additionally, the thorough analysis for Semen Sojae Praeparatum, a Chinese herbal medicine made from fermented black soybeans or soybeans was done for the first time and the characteristic of its HPLC profiling patterns shows the higher content of isoflavone glucosides and aglycones than those of natural soy materials.
- 社団法人日本薬学会の論文
- 2008-01-01
著者
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Yuan Dan
School of Traditional Chinese Medicine, Shenyang Pharmaceutical University
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CHEN Yan
School of Resource and Environment Engineering, East China University of Science and Technology
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KANO Yoshihiro
Institute of Natural Medicine, University of Toyama
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Kano Yoshihiro
Inst. Of Natural Medicine Univ. Of Toyama
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Uno Toshio
Inst. Of Kampo & Healthcare Kanebo Pharmaceuticals Co. Ltd.
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Uno Toshio
Institute Of Kampo & Healthcare Kanebo Pharmaceuticals Co. Ltd.
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PAN Yingni
School of Traditional Chinese Medicine, Shenyang Pharmaceutical University
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ZHANG Shaohui
Institute of Kampo & Healthcare, Kanebo Pharmaceuticals Co., Ltd.
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Pan Yingni
School Of Traditional Chinese Medicine Shenyang Pharmaceutical Univ.
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Zhang Shaohui
Institute Of Kampo & Healthcare Kanebo Pharmaceuticals Co. Ltd.
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Yuan Dan
School Of Traditional Chinese Medicine Shenyang Pharmaceutical University
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Chen Yan
Institute Of Hematology Union Hospital Tongji Medical College Of Huazhong University Of Science And
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Chen Yan
The State Engineering Lab. Of Aids Vaccine Jilin Univ.
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Chen Yan
School Of Computer Wuhan University
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Yuan Dan
School Of Traditional Chinese Medicine Shenyang Pharmaceutical Univ.
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Kano Yoshihiro
Institute Of Natural Medicine University Of Toyama
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