mRNA Expression of Vascular Endothelial Growth Factor-C and -D in Esophageal Squamous Cell Carcinoma
スポンサーリンク
概要
- 論文の詳細を見る
Lymph node metastasis is a major prognostic factor for esophageal cancer patients. However, the molecular mechanisms underlying node metastasis remain unclear. Vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor-D (VEGF-D), which are ligands for VEGFR-3, have been reported to be capable of stimulating lymphangiogenesis under in vivo experimental conditions. The aim of the present study was to measure VEGF-C and/or VEGF-D mRNA expression in clinical specimens of esophageal squamous cell carcinoma, and to examine the correlation between VEGF-C or VEGF-D messenger ribonucleic acid (mRNA) expression and conventional clinicopathological parameters, especially the lymphatic involvement of esophageal squamous cell carcinoma. As a previous study has demonstrated that an expression pattern of high VEGF-C and low VEGF-D is correlated with both lymph node metastasis and lymphatic invasion of cancer cells, we also investigated the VEGF-C/D ratio. Total RNAs were isolated from 51 surgical specimens of esophageal carcinoma tissue and 42 normal esophageal mucosae. The relative mRNA abundance of VEGF-C and VEGF-D was measured by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis, and mRNA expression of VEGF-C and VEGF-D was standardized against glceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression. VEGF-C mRNA was expressed similarly in esophageal carcinoma tissue and normal mucosal tissue ; however, VEGF-D mRNA expression was significantly lower in carcinoma tissue compared with normal mucosal tissue, and therefore the VEGF-C/VEGF-D ratio was significantly increased in tumors compared with normal mucosae. However, neither mRNA expression of VEGF-C or VEGF-D nor the VEGF-C/VEGF-D ratio correlated with any clinicopathological factors, including lymphatic invasion, venous invasion, lymph node status, and tumor stage. VEGF-C and VEGF-D gene expression do not appear to be involved in the lymphatic progression of esophageal carcinoma.
- 新潟大学の論文
著者
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HATAKEYAMA Katsuyoshi
Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental
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Hatakeyama K
Division Of Digestive And General Surgery Niigata University Graduate School Of Medical And Dental S
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Hatakeyama Katsuyoshi
新潟大学 医歯学総合研究科消化器一般外科
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KANDA Tatsuo
Division of Digestive and General Surgery Niigata University Graduate School of Medical and Dental S
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Koyama Yu
新潟大学 第1外科
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Koyama Yu
Division Of Surgery Niigata University Medical And Dental Hospital
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Koyama Y
The First Department Of Surgery Niigata University School Of Medicine
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Koyama Yu
Division Of Digestive And General Surgery Graduate School Of Medical And Dental Sciences Niigata Uni
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Oyamatsu M
Department Of Surgery Niigata University School Of Medicine
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Kanda T
Division Of Digestive And General Surgery Niigata University Graduate School Of Medical And Dental S
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OHASHI MANABU
Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental
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KANEKO Kouji
Division of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata U
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HONMA Hideyuki
Division of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata U
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NAKAGAWA Satoru
Division of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata U
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MARUYAMA Satoshi
Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata University
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Nakagawa Satoru
Division Of Digestive And General Surgery Graduate School Of Medical And Dental Sciences Niigata Uni
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OHTA Tamaki
Department of Pathology
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Hatakeyama K
Division Of Digestive And General Surgery Department Of Surgery Niigata University Graduate School O
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Hatakeyama K
Niigata Univ. Niigata Jpn
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Kanda Tatsuo
Division Of Digestive And General Surgery Department Of Regenerative And Transplant Medicine Niigata
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Kaneko Kouji
Division Of Digestive And General Surgery Graduate School Of Medical And Dental Sciences Niigata Uni
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Ohashi M
Division Of Digestive And General Surgery Department Of Regenerative And Transplant Medicine Niigata
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Honma Hideyuki
Division Of Digestive And General Surgery Graduate School Of Medical And Dental Sciences Niigata Uni
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Hatakeyama Katsuyoshi
Division Of Digestive And General Surgery Graduate School Of Medical And Dental Sciences Niigata Uni
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Koyama Yu
Division Of Digestive And General Surgery Department Of Regenerative And Transplant Medicine Niigata
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Hatakeyama Katsuyoshi
Division Of Digestive And General Surgery Department Of Regenerative And Transplant Medicine Graduat
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Ohashi Manabu
Division Of Digestive And General Surgery Niigata University Graduate School Of Medical And Dental S
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Kimura Tatsuji
Tonen Corporation Research & Development Laboratory
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Hatakeyama Katsuyoshi
The First Department Of Surgery Niigata University School Of Medicine
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Hatakeyama Katsuyoshi
Division Of Digestive And General Surgery Department Of Regeneration And Transplant Medicine Niigata
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