cDNA Cloning and Analysis of the Chicken Homolog of E.coli Endonuclease Ⅲ

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概要

• 論文の詳細を見る

• We have cloned and analyzed the cDNA for the chicken homolog of E. coli endonuclease Ⅲ (chNthll; chicken homolog of E. coli endonuclease Ⅲ-like 1). The cDNA was isolated from a chicken embryonic cDNA library using cDNA of mouse endonuclease Ⅲ (mNthll) as a probe. The obtained　cDNA　was 1211 nucleotide long encoding a protein　consisting of 281 amino acids with a predicted molecular　mass of 32 kDa. The amino acid sequence exhibits 66% and 67%homologies with those of the human　and mouse homologs,　respectively, and the homologies are mostly concentrated　within the C-terminal, 2/3 region of the sequence. The two motifs consisting of a helix-hairpin-helix and an iron-sulphur [4Fe-4S] cluster were also preserved in the chicken homolog suggesting similar enzymatic function in chicken cells as that of endonuclease Ⅲ in E. coli.

• 中国学園大学の論文

著者

• Nakamura Takashi

  Department of Cardiology, Saiseikai Shigaken Hospital

• Seki Shuji

  Departments Of Emergency Medicine And Surgery Okayama University Okayama National Hospital And Kagaw

• Seki Shuji

  Department Of Human Nutrition Faculty Of Contemporary Life Science Chugokugakuen University

• Seki Shuji

  Department Of Biochemistry Cancer Institute Okayama University Medical School

• IKEDA Shogo

  Department of Biochemistry, Cancer Institute, Okayama University Medical School

• Ikeda Shogo

  Department Of Biological Chemistry Okayama University Of Science

• Ikeda Shogo

  Department Of Biochemistry Cancer Institute Okayama University Medical School

• Sarker Altaf

  Department Of Cellular And Molecular Biology Life Science Division Lawrence Berkeley National Labora

• Seki Yuichi

  Tsuyama Medical Examination Center of Chugoku Occupational Health Association

• Nakamura Takashi

  Department Of Molecular Biology Okayama University Graduate School Of Medicine And Dentistry

• Nakamura Takashi

  Department Of Cardiology Saiseikai Shiga Hospital

• H. SarkerAltaf

  Department of Cellular and Molecular Biology, Life Science Division, Lawrence Berkeley National Laboratory, University of California

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