Effects of Heparin and Chemically Modified Heparins on Chlamydia Trachomatis Serovar D Infection of HeLa 229 Cells in Culture

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(Background and Purpose) The prevalence of sexually-transmitted disease caused by Chlamydia trachomatis (C. trachomatis) has greatly increased in recent years especially among young Japanese females, although antibiotics have shown to be effective for such infection. Because it is often asymptomatic, many cases are left untreated. However, there are no valid ways to prevent the chlamydial infection without the proper use of condoms. The process of infection by C. trachomatis is considered to sometimes involve attachment to host genital epithelial cells via host cell surface heparan sulfate. We previously reported that the exogenous addition of heparin and its 2-O-desulfated derivatives (2-ODS heparin) inhibited C. trachomatis serovar L2 infection in a competitive manner. This study was designed to determine in vitro whether C. trachomatis serovar D attachment to HeLa 229 cells is also competitively inhibited by heparan sulfate analogues, heparin and 2-ODS heparin. (Materials and Methods) HeLa 229 cells were inoculated with C. trachomatis serovar D and then incubated with heparin, or its derivatives to assess inhibition of chlamydial infectivity by an inclusion suppression test. (Results) Heparin and 2-ODS heparin showed a significant inhibition of the attachment of C. trachomatis serovar D to host cells, but 6-ODS heparin did not. (Conclusion) Taken together, our previous observations on the same inhibition of C. trachomatis serover L, heparin and 2-ODS heparin can be used for the inhibition of the chlamydial infection. Since the 2-ODS heparin used in this study has no anti-coagulant activity, it may be used in clinical practice with minimal side effects.
愛知医科大学の論文
2006-12-15

Effects of Heparin and Chemically Modified Heparins on Chlamydia Trachomatis Serovar D Infection of HeLa 229 Cells in Culture

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