Inhibition of Potential Lethal Damage Repair and Related Gene Expression after Carbon-ion Beam Irradiation to Human Lung Cancer Grown in Nude Mice
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概要
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Using cultured and nude mouse tumor cells (IA) derived from a human lung cancer, we previously demonstrated their radiosensitivity by focusing attention on the dynamics of tumor clonogens and the early and rapid survival recovery (potential lethal damage repair : PLD repair) occurring after X-ray irradiation. To the authors' knowledge, this is the first study demonstrating gene expression in association with PLD repair after carbon-ion beam or X-ray irradiation to cancer cells. In this study we tried to detect the mechanism of DNA damage and repair of the clonogens after X-ray or carbonion beam irradiation. At first, colony assay method was performed after irradiation of 12 Gy of X-ray or 5 Gy of carbon-ion beam to compare the time dependent cell survival of the IA cells after each irradiation pass. Second, to search the genes causing PLD repair after irradiation of X-ray or carbon-ion beam, we evaluated gene expressions by using semi-quantitative RT-PCR with the selected 34 genes reportedly related to DNA repair. The intervals from the irradiation were 0, 6, 12 and 24hr for colony assay method, and 0, 3, 18 hr for RT-PCR method. From the result of survival assays, significant PLD repair was not observed in carbon-ion beam as compared to X-ray irradiation. The results of RT-PCR were as follows. The gene showing significantly higher expressions after X-ray irradiation than after carbon-ion beam irradiation was PCNA. The genes showing significantly lower expressions after X-ray irradiation rather than after carbon-ion beam irradiation were RAD50, BRCA1, MRE11A, XRCC3, CHEK1, MLH1, CCNB1, CCNB2 and LIG4. We conclude that PCNA could be a likely candidate gene for PLD repair.
- 日本放射線影響学会の論文
- 2007-09-15
著者
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IMAI Takashi
Frontier Res. Ctr., NIRS
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Koyama-saegusa Kumiko
Frontier Research Center National Institute Of Radiological Sciences
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FUJISAWA Takehiko
Department of Thoracic Surgery, Chiba University, Graduate School of Medicine
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Koyama-saegusa Kumiko
Frontier Res. Center National Inst. Of Radiological Sciences
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MIYAMOTO TADAAKI
Research Center Hospital for Charged Particle Therapy, National Institute of Radiological Sciences
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Imai Takashi
Frontier Research Center National Institute Of Radiological Sciences
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YASHIRO Tomoyasu
Department of Thoracic Surgery, Graduate School of Medicine, Chiba University
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Miyamoto Tadaaki
Research Center Hospital For Charged Particle Therapy
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Fujisawa Takehiko
Department Of Surgery Institute Of Pulmonary Cancer Research Chiba University School Of Medicine
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Yashiro Tomoyasu
Department Of Thoracic Surgery Graduate School Of Medicine Chiba University
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Yashiro Tomoyasu
Department Of Thoracic Surgery Graduate School Of Medicine Chiba University : Research Center Hospit
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Imai Takashi
Frontier Res. Ctr. Nirs
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Yashiro Tomoyasu
Department of General Thoracic Surgery, Kimitsu-cho Hospital
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