Growth Phase Dependant Activation of the Precursor of Vibrio mimicus Hemolysin (Pro-VMM)
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概要
- 論文の詳細を見る
Vibrio mimicus (V. mimicus), a causative agent of gastroenteritis and food poisoning, secretes a 63-kDa entero-toxic hemolysin as the most potent virulence factor. The vmhA gene encoding an 83-kDa precursor of the hemolysin was expressed from the early to late log phase of the bacterial growth, and the 79-kDa inactive protoxin was detected from the culture supernatant in the same growth phase. The N-terminal amino acid sequence of the protoxin was determined to be NH_2-Asn-Ile-Ser-Asp-Pro-Val indicating cleavage of the Ala^<25>-Asn^<26> bond by a signal peptidase. In contrast, the hemolytic activity and the mature hemolysin in the culture supernatant were detected only at the late log phase. The maturation of the hemolysin, therefore, is suggested to be achieved by two-step processing, cleavage of the signal peptide followed by the growth phase-dependent removal of the 16-kDa propeptide.
- 公益社団法人日本薬学会の論文
- 2007-08-01
著者
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Miyoshi Shin-ichi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Sakurai Aki
Faculty Of Pharmaceutical Sciences Okayama University
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Takata Noriko
Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Univ.
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Miyoshi Shin-ichi
Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama University
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Okamoto Keinosuke
Faculty Of Pharmaceutical Sciences Okayama University
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Okamoto Keinosuke
Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama University
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Okamoto Keinosuke
Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Division Of Pharmaceutical Science
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Takata Noriko
Faculty Of Pharmaceutical Sciences Okayama University
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Sultan Zafar
Okayama Univ. Okayama Jpn
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Sultan Zafar
Graduate School Of Natural Science And Technology Okayama University
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Mizuno Tamaki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Okamoto Keinosuke
Okayama Univ. Okayama
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Mizuno Tamaki
Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Univ.
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Sultan Zafar
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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