Immobilized Catalyst for Detecting Chemiluminescence in Lipid Hydroperoxide(Analytical Chemistry)
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概要
- 論文の詳細を見る
Both microperoxidase and cytochrome c could be immobilized on a gel for HPLC without losing their activity, the specificity of each immobilized catalyst being similar to that of the free catalyst. With up to 40% of methanol, the chemiluminescence increased as the alcohol concentration increased, while the count decreased with more than 10% methanol for the free cytochrome c. When used as the reactant for flow injection analysis, they do not need to occupy the cell for the emission of chemiluminescence. The immobilized reactant can measure from 400pmol to 10nmol of tert-butylhydroperoxide (t-BuOOH) without staining the emission cell.
- 社団法人日本農芸化学会の論文
- 1991-05-23
著者
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Himeno Michio
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Wadano Akira
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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IKEDA Teturo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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MATUMOTO Motonobu
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Ikeda Teturo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Matumoto Motonobu
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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