Fluorometric Assay of Potential Change of Bombyx mori Midgut Brush Border Membrane Induced by δ-Endotoxin from Bacillus thuringiensis

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概要

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• Bacillus thuringiensis δ-endotoxin, CryIA (a), increased ion permeability of brush border membrane vesicles isolated from midgut epithelia of Bombyx mori larvae. This ion permeability change was measured with a potential-sensitive fluorescent dye, 3,3'-dipropylthiadicarbocyanine iodide. This effect was observed at concentrations of the toxin that correspond to normal effective doses in vivo. CryIA (b) and heat-treat CryIA (a) did not show this effect. CryIA (a) did not show this effect on rat renal brush border membranes. The effects depended on the toxin dose in saturable manner. These suggest that this assay system reflects the mode of action of δ-endotoxin in vivo. The toxin increased various ion permeabilities, suggesting that δ-endotoxin forms non-selective cation pores on brush border membranes.

• 社団法人日本農芸化学会の論文
• 1992-12-23

著者

• Ihara H

  Osaka Prefecture Univ. Osaka Jpn

• Ihara Hideshi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Himeno M

  Osaka Prefecture Univ. Osaka Jpn

• Himeno Michio

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Wadano A

  Dept.applied Biol.chem. Osaka Pref.univ.

• Wadano Akira

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• UEMURA Taro

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Uemura Taro

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

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