A Rapid Isolation of the Unknown 5'-Flanking Sequence of Human CENP-B cDNA with Polymerase Chain Reactions(Biological Chemistry)
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概要
- 論文の詳細を見る
We rapidly and efficiently isolated the 5'-region of cDNA encoding the N-terminal region of human centromere antigen B (CENP-B) including an ATG methionine codon by polymerase chain reactions (PCR). The unknown 5'-flanking sequence of the cDNA was amplified using an adaptor-sequence ligated to the 5' end as a universal primer sequence. To locate the target fragments, we did an additional PCR with another set of two internal primers using samples of the size-fractionated products as templates, rather than using the conventional hybridization procedure. This approach can further be applied to the analysis of other unknown flanking sequences of cDNA or genomic DNA.
- 社団法人日本農芸化学会の論文
- 1991-11-23
著者
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Himeno Michio
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Himeno Michio
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Sugimoto Kenji
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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