Specific Toxicity of δ-Endotoxins from Bacillus thuringiensis to Bombyx mori

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概要

• 論文の詳細を見る

• Two δ-endotoxins, CryIA(a) and CryIA(b), from Bacillus thuringiensis subsp. aizawai were used to investigate the specificity in insecticidal activity. CryIA(a) was 17-fold more toxic to Bombyx mori than CryIA(b). After in vitro solubilization and digestion of these δ-endotoxins, the specificity of toxicity was retained. Trypsin-activated CryIA(a) and CryIA(b) showed specific, high affinity and saturable binding to brush border membrane vesicles (BBMV) from B. mori midguts. These two toxins competed for the same binding site. Dissociation constant for CryIA(a) and CryIA(b) binding to B. mori BBMV was 0.89 nM and 1.46 nM, respectively. In both toxins, dissociation reaction followed a biphasic process with a fast and a very slow component, suggesting that binding of the toxins proceeds through a reversible component and an apparently irreversible component. In the CryIA(a) dissociation reaction, the irreversible component comprised a large portion of total binding. On the other hand in that of CryIA(b), the reversible component was major. These results suggest that the specific toxicity of the toxins to B. mori may depend mainly on irreversibility.

• 社団法人日本農芸化学会の論文
• 1993-02-23

著者

• IHARA Hideshi

  Department of Biological Science, Graduate School of Science, Osaka Prefecture University

• Ihara H

  Osaka Prefecture Univ. Osaka Jpn

• Ihara Hideshi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Himeno Michio

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Kuroda Emi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Himeno M

  Osaka Prefecture Univ. Osaka Jpn

• Himeno Michio

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• WADANO Akira

  Department of Agricultural Chemistry, University of Osaka Prefectrue

• Wadano A

  Dept.applied Biol.chem. Osaka Pref.univ.

• Wadano Akira

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

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