Characterization and Structure of the Cellulase Gene of Bacillus subtilis BSE616(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The Bacillus suhtilis carboxymethyl cellulase (CMCase) gene originally cloned on a 3.2-kb PstI DNA fragment has been localized in a 1.5-kb Sau3AI fragment by a series of subclonings into plasmid pUC19. During the process the promoter region and Shine-Dalgarno (SD) sequence were deleted, but the 1.5-kb insert was shown to direct the synthesis of CMCase in scherichia coli to a high level, probably with the aid of lac promoter. We analyzed the complete nucleotide sequence of the CMCase gene. The CMCase gene is 1500-bp long, encoding a polypeptide of 499 amino acids and a stop codon. The putative "-35" region (TAGACA), "-10" region (TACAAT), and ribosome binding site (RBS) (AAGGAGG) have also been identified in the 5' flanking region. Comparison of the nucleotide sequence to three other published endo-β-1,4-glucanase genes of B. subtilis strains shows that these sequences share very strong homology. It seems that the cellulase genes have been derived from a common ancestor by spontaneous mutation. The probability of carboxy-terminal processing of the CMCase protein is also discussed.
- 社団法人日本農芸化学会の論文
- 1991-02-23
著者
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Park Seung
Department Of Medicine Samsung Medical Center Sungkyunkwan University School Of Medicine
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Park S
Korea Inst. Sci. And Technol. Seoul Kor
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KIM Ha
Department of East-West Medicine, Graduate School, Kyung Hee University
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PACK Moo
Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology
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Pack Moo
Department Of Biological Science And Engineering Korea Advanced Institute Of Science And Technology
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Park Seung
Department Of Anatomy Wonkwang University School Of Medicine
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Kim Ha
Department Of Chemical Engineering Hanyang University
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Kim Ha
Department Of Biological Science And Engineering Korea Advanced Institute Of Science And Technology
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Park Seung
Department Of Anatomy College Of Medicine Konkuk University
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