Interspecific Hybridization of Aspergillus awamori var. kawachi and Aspergillus oryzae by Protoplast Fusion(Microbiology & Fermentation Industry)

概要

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A new breeding technique for Aspergillus awamori var. kawachi and Aspergillus oryzae involving protoplast fusion was studied to obtain more useful interspecific hybrids for making spirits for drinking. A yield of protoplasts from mycelia of these fungi was obtained using a Trichoderma viride BIA lytic enzyme system. Interspecific hybridization between these fungi was brought about by protoplast fusion, in the presence of 35% polyethylene glycol as a fusogen. A strain obtained from the heterokaryon using d-camphor showed high stability and numerous sporulation, producing the same levels of amylase and citric acid as those of the original strains. The strain also showed a 2-fold DNA content per nucleus and about 1.5-fold conidial diameter compared to those of the original strains. The new strain was assumed to be a heterozygous diploid. Some strains considered to be recombinants were spontaneously obtained at low frequency from the heterozygous diploids. The existence of a parasexual cycle in these fungi was also discovered using protoplast fusion.
社団法人日本農芸化学会の論文
1988-08-23