The Modes of Action of Three Xylanases from Mesophilic Fungus Strain Y-94 on Xylooligosaccharides(Biological Chemistry)
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概要
- 論文の詳細を見る
The modes of action of theree thermostable endo-xylanases (Xn-A, Xn-B, and Xn-C) from the mesophilic fungus strain Y-94 on xylooligosaccharides and their alditols (DP 2〜8) were studied. Non of the enzymes could hydrolyse xylobiose. Oligosaccharides upwards from xylotetraose were immediately hydrolysed by the endo-xylanases, but xylotriose was slowly hydrolysed. Xn-A hydrolysed xylotriose more slowly than the other two. From the dependency of the molecular activity (ko) on the chain length of the substrates, it was suggested that the three xylanases had the same subsite size (5 xylose units). Analysis of the frequency distribution of bond cleavage of oligosaccharide-alditols showed that no enzymes could attack the first bond from the non-reducing end of oligosaccharides. The other bonds were hydrolysed by these enzymes by endo-type action. The action pattern for xylopentaitol suggested that the catalytic site was located between the second and third subsite from the non-reducing end, since the substrate was mainly hydrolysed to xylobiose and xylotriitol.
- 社団法人日本農芸化学会の論文
- 1988-04-23
著者
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Takasaki Yoshiyuki
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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Mitsuishi Yasushi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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YAMANOBE Takashi
Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of Internatio
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YAGISAWA Mitsuo
Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of Internatio
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Mitsuishi Y
Agency Of Industrial Science And Technology Ministry Of International Trade And Industry Ibaraka Jpn
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Yagisawa M
Agency Of Industrial Science And Technology Ministry Of International Trade And Industry Tsukuba‐shi
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Yagisawa Mitsuo
Fermentation Research Institute Agency Of Industrial Science & Technology
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Yamanobe T
Agency Of Industrial Science And Technology Ministry Of International Trade And Industry Ibaraka Jpn
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Yamanobe Takashi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
関連論文
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- Purification and Some Properties of a Microcrystalline Cellulose-hydrolyzing Enzyme (Avicelase II) from Fungal Strain Y-94(Microbiology & Fermentation Industry)
- The Modes of Action of Three Xylanases from Mesophilic Fungus Strain Y-94 on Xylooligosaccharides(Biological Chemistry)
- Purification and Properties of Thermostable Xylanases from Mesophilic Fungus Strain Y-94(Bioligical Chemistry)
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