Some Enzymatic Properties of Endo-1,4-β-glucanase Components from Fungal Strain Y-94(Microbiology & Fermentation Industry)
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概要
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The enzymatic properties of several purified endo-1,4-β-glucanase components [1,4-β-D-glucan glucanohydrolase (EC 3.2.1.4)] from a fungus, Y-94, were investigated. The pH and temperature optima of the enzymes were from 4.5-5.5 and 50-70℃ and their stabilities for various pHs and temperatures were observed over a wide range of 3.0-8.5 and 50-80℃, respectively. All the endo-1,4-β-glucanase components were strongly inactivated by Hg^<2+> and Mn^<2+> and several of them were activated by the addition of sodium dodecyi sulfate (SDS) when carboxymethyl cellulose (CMC) was used as a substrate. Most purified endo-1,4-β-glucanase components could hydrolyze CMC, hydroxyethyl-cellulose (HEC), phosphoric acid swollen-cellulose (PSC), and xyloglucan. Their Michaelis constants (Km) for CMC [average degree of polymerization (<DP>^^^-)=1050] were from 0.06-0.28% and for the cellooligosaccharides cellotetraose and cellopentaose, from 1.20-6.75mM and 0.48-1.09mM, respectively. The synergistic effect, generated by a combination of the endo-1,4-β-glucanase components, avicelases, and β-glucosidase, resulted in a 1.3-2.4-fold increase of Avicel-, CMC-, and cellulose powder-hydrolyzing activities, compared to the sum of the activities of the individual enzymes regarding these substrates. No synergism was observed within the endo-1,4-β-glucanase components. Increasing the molecular weight of cellooligosaccharide that was used as a substrate tended to increase the hydrolyzing activities for them of the enzyme components.
- 社団法人日本農芸化学会の論文
- 1990-02-23
著者
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Mitsuishi Yasushi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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YAMANOBE Takashi
Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of Internatio
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Yamanobe Takashi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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Yamanobe Takashi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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Mitsuishi Yasushi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
関連論文
- Purification and Properties of a β-Glucosidase from Fungal Strain Y-94(Microbiology & Fermentation Industry)
- Purification of Endo-1,4-β-glucanase Components from Fungal Strain Y-94(Microbiology & Fermentation Industry)
- Some Enzymatic Properties of Endo-1,4-β-glucanase Components from Fungal Strain Y-94(Microbiology & Fermentation Industry)
- Purification and Some Properties of a Microcrystalline Cellulose-hydrolyzing Enzyme (Avicelase II) from Fungal Strain Y-94(Microbiology & Fermentation Industry)
- The Modes of Action of Three Xylanases from Mesophilic Fungus Strain Y-94 on Xylooligosaccharides(Biological Chemistry)
- Purification and Properties of Thermostable Xylanases from Mesophilic Fungus Strain Y-94(Bioligical Chemistry)
- Isolation of a Cellulolytic Enzyme Producing Microorganism, Culture Conditions and Some Properties of the Enzymes(Microbiology & Fermentation Industry)
- Improvement of Fungal Strain Y-94, a Cellulolytic Enzyme Hyperproducer, and Enzymatic Saccharification of Exploded Wood(Microbiology & Fermentation Industry)
- Identification of Nitrogen-fixing Hydrogen Bacterium Strain N34 and Its Oxygen-resistant Segregant Strain, Y38