Purification and Properties of Thermostable Xylanases from Mesophilic Fungus Strain Y-94(Bioligical Chemistry)
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概要
- 論文の詳細を見る
A mesophilic fungal strain Y-94 produced three types of thermostable endo-xylanases accompanied by the formation of a large amount of cellulase. These xylanases were separated from the cellulase by heat treatment at 65℃ for 2.5 hr and purified by DEAE-Toyopearl chromatography, chromatography on an anion exchanger (PBE 94), and Bio-Gel A 0.5 m gel filtration. The molecular weights of the three types of xylanase, designated as xylanase A, B and C, were 51,000, 48,000, and 35,000, respectively. All three enzymes showed highest activity at pH 4.9 and 80℃ in 10min of incubation, and had the same hydrolysis pattern of larch wood xylan with the end-products of xylobiose and xylose. Thus their activities appear essentially the same but not their stabilities. Xylanase A and B were stable from pH 2.5 to 9.0 but xylanase C was unstable above pH 5.5. Xylanase C was unstable at 70℃ where other two were stable.
- 社団法人日本農芸化学会の論文
- 1987-12-23
著者
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Takasaki Yoshiyuki
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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Takasaki Yoshiyuki
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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Mitsuishi Yasushi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
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YAMANOBE Takashi
Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of Internatio
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YAGISAWA Mitsuo
Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of Internatio
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Mitsuishi Y
Agency Of Industrial Science And Technology Ministry Of International Trade And Industry Ibaraka Jpn
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Yagisawa M
Agency Of Industrial Science And Technology Ministry Of International Trade And Industry Tsukuba‐shi
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Yagisawa Mitsuo
Fermentation Research Institute Agency Of Industrial Science & Technology
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Yamanobe T
Agency Of Industrial Science And Technology Ministry Of International Trade And Industry Ibaraka Jpn
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Yamanobe Takashi
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
関連論文
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