Pullulanase-Amylase Complex Enzyme from Bacillus subtilis(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
A novel pullulanase-amylase complex enzyme, which hydrolyzes pullulan into maltotriose as well as starch into maltose and maltotriose as the main products, was found in the culture filtrate of a strain of Bacillus subtilis newly isolated from soil. The enzyme was purified to almost complete homogeneity by means of calcium phosphate gel absorption, DEAE-Sepharose column chromatography and Bio-gel A-1.5m filtration. The optimum pH of the pullulanase activity was observed at around 7.0 to 7.5, with a discernible shoulder around pH 5.0. While the optimum pH of the amylase activity was 6〜7. The optimum temperatures of the pullulanase and amylase activities were about 60℃ and about 50℃, respectively. The molecular weight was estimated to be about 450,000 by the gel filtration method. The enzyme could be used for the production of glucose from starch with glucoamylase and the production of a new type of syrup containing a relatively high amount of meltotriose, 50〜55%, from starch.
- 社団法人日本農芸化学会の論文
- 1987-01-23
著者
-
Takasaki Yoshiyuki
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
-
Takasaki Yoshiyuki
Fermentation Research Institute Agency Of Industrial Science And Technology Ministry Of Internationa
-
Yamanobe T
Agency Of Industrial Science And Technology Ministry Of International Trade And Industry Ibaraka Jpn
関連論文
- The Modes of Action of Three Xylanases from Mesophilic Fungus Strain Y-94 on Xylooligosaccharides(Biological Chemistry)
- Purification and Properties of Thermostable Xylanases from Mesophilic Fungus Strain Y-94(Bioligical Chemistry)
- Isolation of a Cellulolytic Enzyme Producing Microorganism, Culture Conditions and Some Properties of the Enzymes(Microbiology & Fermentation Industry)
- Biodegradation of Phenylmercuric Acetate by Organomercury-resistant Penicillium sp. MR-2(Microbiology & Fermentation Industry)
- Pullulanase-Amylase Complex Enzyme from Bacillus subtilis(Microbiology & Fermentation Industry)
- Production of Maltohexaose by α-Amylase from Bacillus circulans G-6