Production and Purification of New Maltohexaose-forming Amylases from Alkalophilic Bacillus sp. H-167(Microbiology & Fermentation Industry)
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概要
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Three α-amylases that produce maltohexaose as the main product from starch were found in a culture filtrate of alkalophilic Bacillus sp. H-167, which was isolated from soil. The optimum culture conditions for enzyme production were determined: initial medium pH 9.4; temperature, 37℃; and 50〜60 hr cultivation under aerobic conditions. The enzymes were separated completely and purified to be homogeneous on disc electrophoresis by using chromatographies on DEAE-Toyopearl 650M and DEAE-Sepharose CL-6B, followed by gel filtration on Sephadex G-100 or Ultrogel AcA-34. All the enzymes produced maltohexaose in the early stage of hydrolysis, the maximum yield being 25〜30%.
- 社団法人日本農芸化学会の論文
- 1988-02-23
著者
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Hayashi T
The Riken Institute
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HORIKOSHI Koki
The Riken Institute
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HAYASHI Takaya
The Riken Institute
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AKIBA Teruhiko
The Riken Institute
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