Selective Excretion of Alkaline Xylanase by Escherichia coli Carrying pCX311
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概要
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Plasmid pCX311, which we constructed, has two HindIII DNA fragments (2.6kbp and 2.Qkbp) of alkalophilic Bacillus sp. strain C-125 in the HindIII site of pBR322. These two fragments were essential not only for the xylanase production but also for the excretion of periplasmic proteins. The cloned 4.6 kbp fragment encodes some components that made the outer membrane of E. coli permeable. Some proteins such as xylanase and β-lactamase were excreted, but alkaline phosphatase was not excreted into the culture broth.
- 社団法人 日本農芸化学会の論文
著者
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HONDA Hiroshi
The Riken Institute
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KUDO Toshiaki
The Riken Institute
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HORIKOSHI Koki
The Riken Institute
関連論文
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