Simultaneous Qualitation and Quantification of Thirteen Bioactive Compounds in Flos Lonicerae by High-Performance Liquid Chromatography with Diode Array Detector and Mass Spectrometry
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概要
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A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of thirteen bioactive compounds in Flos Lonicerae. The optimal chromatographic conditions were obtained on a C_<18> column (250×4.6mm, 5.0μm) with the column temperature at 30℃. The mobile phase was composed of(A) acetic acid aqueous (0.4%, v/v) and (B) acetonitrile using a gradient elution, the flow rate was 1 ml/min. Detection wave lengths were set at 240 nm for iridoids (loganin, sweroside, secoxyloganin and centauroside), 330 nm for phenolic acids (chlorogenic acid, caffeic acid, 4,5-di-O-caffeoyl quinic acid and 3,4-di-O-caffeoyl quinic acid) and 360 nm for flavonoids (rutin, hyperoside, quercetin-3-O-glucoside, luteolin-7-O-glucoside and lonicerin). The identities of the peaks were accomplished by comparing retention times, UV and mass data with reference compounds under the same conditions. All calibration curves showed good linear regression (r^2>0.9983) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.78-1.85% and 1.13-2.36%, respectively, and the overall recoveries of 91.3-104.2% for the thirteen compounds analyzed. The verified method was successfully applied to quantitative determination of the three types of bioactive compounds in ten commercial Flos Lonicerae samples from different markets in China. The analytical results demonstrated that the contents of the thirteen analytes were relatively variant.
- 公益社団法人日本薬学会の論文
- 2007-07-01
著者
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Tang Dan
Department of Gastroenterology, Changzheng Hospital, Second Military Medical University
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QIAN Zheng-Ming
Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education
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LI Hui-Jun
Department of Pharmacognosy, China Pharmaceutical University
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LI Ping
Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education
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Li Ping
Key Laboratory Of Modern Chinese Medicines Ministry Of Education China Pharmaceutical University:dep
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Li Ping
Key Laboratory Of Modern Chinese Medicines (china Pharmaceutical University) Ministry Of Education:d
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Tang Dan
Key Laboratory Of Modern Chinese Medicines Ministry Of Education China Pharmaceutical University
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Li Hui-jun
中華人民共和国
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Li Hui-jun
Key Laboratory Of Modern Chinese Medicines (ministry Of Education) China Pharmaceutical University
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Qian Zheng-ming
Key Laboratory Of Modern Chinese Medicines (china Pharmaceutical University) Ministry Of Education
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Li Hui-jun
Department Of Pharmacognosy China Pharmaceutical University
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REN Mei-Ting
Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education
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Ren Mei-ting
Key Laboratory Of Modern Chinese Medicines (china Pharmaceutical University) Ministry Of Education
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Li Ping
Key Lab. Of Heavy Ion Radiation Biology And Medicine Of Chinese Acad. Of Sciences
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