培養細胞を用いた環境汚染物質の毒性評価-有機錫化合物と微量金属類について-

概要

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The purpose of this study is to research the toxicity assessments of environmental pollutants using cultured cells, having whether or not these compounds take some damages in cellular levels. Some cultured leukemia cells were used as a model system to assess the cytotoxicity of various organotin compounds and trace metals. The results of these studies in vitro suggest the following: 1. Judging from LC_<50> values, organometallic compounds were more toxic than that of the trace metals. 2. The viability of cells treated with Se decreased at concentration of 10 μM, as compared with those of other trace metals. The viability of cells treated with Pb did not exhibited similar change at concentration of 1,000 μM. 3. Dibutyltin or tributyltin compounds was observed to be more toxic than the tetrabutyltin or mono-butyltin compounds of the same chain length. 4. Tripropyltin compounds were more toxic than other alkyltin compounds. 5. A difference in toxicity for butyltin compounds between Molt-4 cells and HL-60 cells was observed, the former to be weak than the latter. These results indicate that the cultured cells can be applied to short-term toxicity assessments of environmental pollutants.
順天堂大学の論文