Prostaglandin E Receptor EP4 Antagonist Suppresses Lipopolysaccharide-Induced Osteoclast Formation and Inflammatory Bone Loss
スポンサーリンク
概要
- 論文の詳細を見る
Prostaglandin E_2 (PGE_2) is mainly produced by osteoblasts in bone tissue, and acts as a potent stimulator of bone resorption. In osteoblasts, PGE_2 production was greatly stimulated by lipopolysaccharide (LPS) following the expression of cyclooxygenase (COX)-2 and membrane-bound PGE synthase (mPGES)-1 mRNA. In the coculture of mouse bone marrow cells and osteoblasts, LPS induced PGE production and osteoclast formation, and EP4 antagonist completely suppressed osteoclast formation, indicating that the PGE_2-mediated EP4 signal is essential for LPS-induced osteoclast formation. Inflammatory bone diseases including periodontitis are known to be accompanied by bone loss with increased osteoclastogenesis. To examine the role of EP4-mediated PGE_2 action in periodontitis, we examined the effects of EP4 antagonist on LPS-induced bone resorption using mouse alveolar bone. In an organ culture of alveolar bone, LPS-induced bone resorbing activity and EP4 antagonist suppressed this LPS-induced bone resorption. In an experimental model of periodontitis, LPS was injected into the lower gingiva, and the bone mineral density of alveolar bone was measured. LPS-induced the loss of alveolar bone, which was recovered by the treatment with EP4 antagonist in vivo. Therefore, EP4 antagonist is a possible candidate for the therapy of inflammatory bone disease including periodontitis.
- 2007-04-01
著者
-
Matsumoto Chiho
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
-
Takita Morichika
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
-
Inada Masaki
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
-
Maruyama Takayuki
Discovery Research Laboratories I, Minase Research Institute, Ono Pharmaceutical Co., Ltd.
-
Miyaura Chisato
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
-
Inada Masaki
Dep. Of Biotechnology And Life Sci. Tokyo Univ. Of Agriculture And Technol.
-
Miyaura Chisato
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Maruyama Takayuki
Discovery Research Laboratories I Minase Research Institute Ono Pharmaceutical Co. Ltd.
-
Takita Morichika
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Inada Masaki
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Matsumoto Chiho
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Miyaura C
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
MIYAURA CHISATO
Department of Biochemistry, School of Dentistry, Showa University
関連論文
- Role of Prostaglandin E in Receptor Activator of Nuclear Factor-κB Ligand (RANKL) Expression in Osteoblasts Induced by Cell Adhesion to Bone Marrow B-lymphocytes
- Prostaglandin E Receptor EP4 Antagonist Suppresses Lipopolysaccharide-Induced Osteoclast Formation and Inflammatory Bone Loss
- Nobiletin, a Polymethoxy Flavonoid, Suppresses Bone Resorption by Inhibiting NFκB-Dependent Prostaglandin E Synthesis in Osteoblasts and Prevents Bone Loss Due to Estrogen Deficiency
- Involvement of EMMPRIN/CD147 in pericellular proteolysis by augmenting the production of MMP-1 and localization of MMP-1 on tumor cell surface
- Cell Shape and Matrix Production of Fibroblasts Cultured on Fibroin-organized Silk Scaffold with Type-II β-turn Structured (Ala-Gly-Ala-Gly-Ser-Gly)n Sequences
- Suzaku Observations of the Hard X-Ray Variability of MCG -6-30-15 : the Effects of Strong Gravity around a Kerr Black Hole
- Suzaku Observation of 1H0707-495 : Puzzling Spectral Drop around 7keV(Chapter 10. Super Massive Black Holes, The Extreme Universe in the Suzaku Era)
- Soybean isoflavones preserve bone mass in hindlimb-unloaded mice
- Discovery of Short-Timescale Variability of Seyfert 2 Galaxy NGC 6300 with XMM-Newton(Poster)
- Effect of Cu on the Corrosion Resistance of a NiMo_2B_2-Dispersed Ni-Based Alloy
- 1α,25-Dihydroxyvitamin D_3-26,23-lactam, a Novel Vitamin D_3 Analog, Acts as a Vitamin D_3 Antagonist in Human Prostate Cancer Cells
- Capsaicin, A Ligand for Vanilloid Receptor-1, Transduces Suppressive Signal for Osteoclast Differentiation in Bone
- B-Lymphocytes are Elevated in Mouse Bone Marrow by Estrogen Deficiency, and Induce Receptor Activator of Nuclear Factor κB Ligand (RANKL) Expression in Osteoblasts via Cell Adhesion
- Dietary Bisphenol A Suppresses the Growth of Newborn Pups by Insufficient Supply of Maternal Milk in Mice
- Naringin Suppresses Osteoclast Formation and Enhances Bone Mass in Mice
- Failure to Demonstrate the Stimulative Effect of Calcitonin on Cyclic AMP Accumulation in Avian Bone in Vitro
- Kinetics of Change in Casein Aggregation as a Result of Solvent-Induced Structural Change
- Nobiletin, a Polymethoxy Flavonoid, Suppresses Bone Resorption by Inhibiting NFκB-Dependent Prostaglandin E Synthesis in Osteoblasts and Prevents Bone Loss Due to Estrogen Deficiency
- モデル動物を用いた閉経後骨粗鬆症と炎症性歯周病の関係解明
- Polymethoxy Flavonoids, Nobiletin and Tangeretin, Prevent Lipopolysaccharide-Induced Inflammatory Bone Loss in an Experimental Model for Periodontitis
- モデル動物を用いた閉経後骨粗鬆症と炎症性歯周病の関係解明
- Polymethoxy Flavonoids, Nobiletin and Tangeretin, Prevent Lipopolysaccharide-Induced Inflammatory Bone Loss in an Experimental Model for Periodontitis
- Discovery of Short-Timescale Variability of Seyfert 2 Galaxy NGC 6300 with XMM-Newton(Poster)
- Bombyx mori silk fibroin scaffolds for bone regeneration studied by bone differentiation experiment(BIOMEDICAL ENGINEERING)
- Genetic Analysis of the rat Na-K-2Cl Cotransporter (NKCC2) Gene in SHRSP/Izm:Positional Candidate Approach
- The Protective Effects of β-Cryptoxanthin on Inflammatory Bone Resorption in a Mouse Experimental Model of Periodontitis