Bone Engineering Using Human Demineralized Dentin Matrix and Recombinant Human BMP-2(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
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概要
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We first confirmed the osteoinductive property of human demineralized dentin matrix (DDM) histologically. Human DDM was prepared from vital, extracted teeth and implanted into the subcutaneous tissue in nude mice. The shape of the DDM was a particle type and its size varied from 0.4 to 0.8mm. The hard tissue induction by 70mg of the DDM was estimated at 4 weeks after implantation. The DDM induced bone and cartilage independently. In addition, the time-course of the bioassay by recombinant human BMP-2 (5.0μγ)/DDM (70mg) was estimated in rat subcutaneous tissues. Histological examination showed that the BMP-2/DDM induced bone and cartilage and the DDM were gradually replaced by new bone. The morphometric analysis demonstrated that the BMP-2/DDM showed 79.0% in the volume of bone and marrow, and 21.0% in that of DDM at 32 weeks. These results indicate that human DDM particles are osteoinductive matrics and the DDM might be effective as a carrier of BMP-2 for bone engineering.
- 硬組織再生生物学会の論文
著者
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MURATA Masaru
Department of Oral Maxillo-facial Surgery, School of Dentistry, Health Sciences University of Hokkai
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Murata Masaru
Department Of Architecture And Building Engineering School Of Engineering
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Murata Masaru
Department Of Oral And Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokka
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