Inhibition of Angiogenesis and HIF-1α Activity by Antimycin A1(Molecular and Cell Biology)
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概要
- 論文の詳細を見る
We identified antimycin A1 as an inhibitor of the hypoxia-response element (HRE) from screening using a reporter under the control of HRE under hypoxic conditions. Antimycin A1 was effective at 20pg/ml in inhibiting the reporter activity. The expression of vascular endothelial growth factor (VEGF) mRNA during hypoxia was also inhibited by antimycin A1. Angiogenesis induced by implantation of mouse sarcoma-180 cells was significantly inhibited by non-toxic doses of antimycin A1. Hypoxia inducible factor (HIF)-1α protein levels were significantly decreased by antimycin A1, but its mRNA level was not affected. Antimycin A1 is known to be an inhibitor of mitochondrial electron transport system, and depletion of mitochondria abolished antimycin A1-effect, at least in part. Inhibitors of proteasome or protein synthesis did not affect the decrease in HIF-1α level induced by antimycin A1. These results indicate that antimycin A1 inhibited angiogenesis through decrease in VEGF production caused by inhibition of HIF-1α activation.
- 公益社団法人日本薬学会の論文
- 2006-07-01
著者
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MAEDA Masayuki
Department of Radiology, Mie University School of Medicine
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NOSE Kiyoshi
Department of Microbiology, Showa University School of Pharmacy
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SHIBANUMA Motoko
Department of Microbiology, Showa University School of Pharmacy
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HASEBE Yuki
Department of Microbiology, Showa University of Pharmaceutical Sciences
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EGAWA Kiyoshi
Department of Microbiology, Showa University of Pharmaceutical Sciences
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Hasebe Yuki
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Nose K
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Nose K
Showa Univ. Pharmaceutical Sci. Tokyo Jpn
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Nose Kiyoshi
Department Of Microbioilogy Showa University School Of Pharmaceutical Sciences
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Shibanuma Motoko
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Nose Kiyoshi
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Egawa K
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Maeda Masayuki
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Egawa Kiyoshi
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Maeda Masayuki
Department Of Agricultural Chemistry Faculty Of Agriculture Yamagata University
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