Induction of p16^<INK4a> Transcription and of Cellular Senescence by Aclacinomycin-Derivatives and Cardiac Glycosides
スポンサーリンク
概要
- 論文の詳細を見る
Stable transformants of Saos-2 cells that contain the luciferase reporter gene under the control of the human p16^<INK4a> transcriptional regulatory region were established, and were used to identify growth-inhibiting substances from culture broths of actinomycetes and extracts of plants. Among the active substances so far identified were an aclacinomycin-derivative, cenerubin B, and a cardiac glycoside, periplocin. These substances inhibited growth of normal human fibroblasts, and induced senescent phenotypes including a flattened morphology and increased acidic β-galactosidase activity, although the activities of their derivatives to induce p16^<CDKN2> and β-galactosidase did not coincided with each other. These results suggest that the reporter system using the p16^<CDKN2> transcriptional regulatory region is a useful means for screening growth inhibiting substances that are potential anti-tumor agents.
- 公益社団法人日本薬学会の論文
- 2002-04-01
著者
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Kurihara Youji
School of Pharmaceutical Sciences, Kitasato University
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Kurihara Y
School Of Pharmaceutical Sciences Kitasato University
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NOSE Kiyoshi
Department of Microbiology, Showa University School of Pharmacy
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EGAWA Kiyoshi
Department of Microbiology, Showa University of Pharmaceutical Sciences
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Nose K
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Nose K
Showa Univ. Pharmaceutical Sci. Tokyo Jpn
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Nose Kiyoshi
Department Of Microbioilogy Showa University School Of Pharmaceutical Sciences
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KURIHARA Yosuke
Department of Microbiology, Showa University School of Pharmaceutical Sciences
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ITO Tatsuya
Pharmaceutical Technology Laboratory, Chugai Pharmaceutical Co., Ltd.
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MATSUMOTO Masahiko
Pharmaceutical Technology Laboratory, Chugai Pharmaceutical Co., Ltd.
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Nose Kiyoshi
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Egawa K
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Egawa Kiyoshi
Department Of Microbiology Showa University Of Pharmaceutical Sciences
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Matsumoto Masahiko
Pharmaceutical Technology Laboratory Chugai Pharmaceutical Co. Ltd.
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