ヒト乳腺上皮細胞による補体D因子産生(自然科学系)

概要

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Complement components were assayed in culture supernatants of normal human mammary epithelial cells (HMEC) and 3 kinds of mammary cancer-derived cell lines, MRK-nu-1, YMB-1 and YMB-1 E. When these cells were cultured In medium without addition of any stimulator for 3 days, Clq, C4, C3 and factor D were clearly detected In the supernatants of all these cells. Further studies were carried out to analyze factor D production by these cells. Mammary cancer-derived cell lines, as well as normal HMEC, produced and released 0.35-1.72 ng of factor D/l0^6cells/day in the supernatants. When IFN (interferon)-γ was added in culture medium, the factor D level in the supernatants increased in a dose-dependent manner. Addition of histamine or LPS (lipopolysaccharide) did not significantly effect the factor D level. Factor D production by these cells was inhibited by the presence of 1.0μg/mL of cycloheximide reversibly, indicating de novo synthesis of factor D by these cells. This is the first report on factor D synthesis by mammary epithelial cells.
大阪府立大学の論文
1999-01-29