正常ヒト肝細胞による補体D因子産生(予報)(自然科学系)

概要

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The biosynthesis of complement components by normal human hepatocytes was studied in vitro. In culture supernatants of normal hepatocytes [human primary hepatocytes (epithelial) cells] , many complement components including factor D were distinctly detected by immunoblot analysis. In our studies focusing on factor D production by these cells, we have found the following: (i) normal hepatocytes produced increasing amounts of factor D over a 5-day period; (ii) when hepatocytes were cultured for 5 days without addition of any stimulator, 331.1±41.38ng (/10^8 cells, n=12) of factor D was secreted; (iii) addition of TNF-α, IFN-γ, IL-1β or LPS in the culture medium did not show any distinct effect on the amounts of secreted factor D; and (iv) reversible inhibition of factor D secretion was observed when the cells were cultured in the presence of cycloheximide, indicating that factor D is synthesized de novo. These findings suggest the possibility of factor D synthesis by the liver in vivo, to be contradictory to the previous reports that factor D is identical to adipsin secreted by adipocytes in adipose tissue but not by the liver.
大阪府立大学の論文
2000-03-27