Immuno-histochemical Differentiation of Fiber Type in Human Skeletal Muscle Using Polyclonal Antibody against Troponin I Subunit
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概要
- 論文の詳細を見る
Troponin I (TN-I) is a regulatory protein that differs between slow and fast muscle due to its molecular components. In this study, an antibody demonstrating cross-reactivity with human skeletal TN-I was obtained from antisera against chicken breast muscle TN-I (fast TN-I). Human, chicken and rabbit myofibrils treated with the antibody were observed by electron microscopy. Striated patterns of reacted antibody were observed on actin filaments wherein the interval between striations was 32-35 nm in each specimen. Immunofluorescent staining of human skeletal muscle with the antibody showed that positive staining muscle fibers correspond to type II fibers of ATPase staining. Similar findings were obtained in the case of muscles affected by human neuromuscular diseases. These results indicate that the antibody is a pure polyclonal antibody to fast TN-I thus making it useful in clinical and immunochemical research.
- 北里大学の論文
- 1995-12-31
著者
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Nakamura Takeshi
Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine
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Matsumoto Nobuko
Internal Medicine Sagamihara Kyodo Hospital:department Of Internal Medicine Kitasato University Scho
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Nakamura Takeshi
Department Of Cardiovascular Medicine Kyoto Prefectural University School Of Medicine
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Yasui Yoshie
Department of Neurosurgery, Kitasato University School of Medicine
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Torii Junzo
Department of Internal Medicine, Kitasato University School of Medicine
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Yasui Yoshie
Department Of Neurosurgery Kitasato University School Of Medicine
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Yasui Yoshie
Department Of Section Of Integrated Clinical Research Kitasato University School Of Medicine
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Torii Junzo
Department Of Internal Medicine Kitasato University School Of Medicine
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Nakamura Takeshi
Department Of Agricultural Chemistry The University Of Tokyo
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Nakamura Takeshi
Department Of Parasitology Kitasato University School Of Medicine
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Nakamura Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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