マウス初代軟骨細胞におけるbone morphogenetic protein-2 のヒアルロン酸合成酵素発現制御

概要

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Bone morphogenetic proteins (BMPs) were originally identified in demineralized bone matrix as a protein inducing bone formation in vivo. BMPs form a large family of morphogens for cartilage and play a critical role in bone morphogenesis. BMP-2 was first cloned and expressed by Wozney and co-worker. Recombinant BMP-2, BMP-4, and BMP-7 induce cartilage differentiation in vivo and chondrogenesis of chick limb bud mesodermal cells in vitro. BMP-4 and BMP-7 can maintain the proteoglycan synthesis in chemically defined serum-free medium. The biosynthesis of hyaluronan (HA) is a key step in chondrogenesis. Hyaluronan synthases (HAS1, 2 and 3) are functionally critical in biosynthesis of HA. In the present study, we have investigated the potential actions of recombinant human BMP2 on HA biosynthesis in mouse primary chondrocytes. The results revealed a reciprocal regulation of HAS1 and HAS2 by BMP-2. HAS2 was stimulated by BMP-2 while concurrently there was a down regulation of HAS1. There appear to be no effect of BMP-2 on HAS3.
愛知医科大学の論文
2005-03-10