生物工学を支える材料の基礎研究とその細胞培養への応用

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To develop a high-function animal cell culture substratum, a dendrimer-modified substratum was examined in hepatocyte culture. By immobilizing fructose-modified dendrimers on a polystyrene culture plate (fructose-dendrimer), it proved possible to maintain the number of initially adhered hepatocytes and the urea synthesis activity. It was also found that hepatocytes formed spheroids when cultured on fructose-dendrimer. However, the adhesion was not sufficiently strong and the spheroids detached easily into the culture medium. Galactose, which is a ligand for the asialoglycoprotein receptor on the hepatocyte cytoplasmic membrane, was chosen as another ligand for modification in order to maintain adhesion of spheroids for long periods. Simultaneous modification of dendrimers with fructose and galactose had a marked effect on spheroid adhesion. Suppression of apoptosis and necrosis was observed in hepatocyte spheroids cultured on a dendrimer modified with fructose and galactose (F/G dendrimer). Moreover, the hepatocyte spheroids cultured on the F/G dendrimer had higher activity in liver-specific functions, such as urea synthesis and albumin gene expression, than those cultured on single ligand-modified dendrimers. Immobilization on mixed-ligand-modified dendrimers was thus able to generate a suitable surface for hepatocyte spheroid formation. These dendrimers could serve as a powerful tool for generating custom-made scaffolds for cells other than hepatocytes if suitable ligands were selected for each cell type. This surface-modification technology promises to contribute to the development of tissue engineering.
社団法人日本生物工学会の論文
2006-02-25

生物工学を支える材料の基礎研究とその細胞培養への応用

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