周産期における各種体液中のSAAならびにCRPの定量・定性解析と免疫生物学的意義

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Serum amyloid A protein (SAA) and C-reactive protein (CRP) are one of the human plasma proteins that have been recognized as an important host defense molecule. It is reported that SAA is produced with the mammary gland which is an extrahepatic producing tissue in various mammals except human and being secreted into colostrum. We have previously made clear that CRP is included in human colostrum and also what an automated commercially available latex-enhanced assay is effective to explore SAA in human colostrum and to measure its concentration. Consequently, the immunobiological significance of SAA and CRP contained in human colostrum has been of major concern. In this study, we examined to measure of these concentration by the high sensitive assay and to investigate the molecular attributes by western blotting. SAA was contained in the all of breast milk obtained from seventeen cases available. The mean concentration of SAA included in the breast milk increased from 0.135±0.33 μg/ml of the childbirth day to 1.494±1.17μg/ml in the observed puerperal period. There was a peak of the level of SAA on the third puerperium day. It was in contrast with the level of the milk CRP having reached a peak on the second puerperium day, and both levels of the blood SAA and CRP having become a peak on the first puerperium day. The molecular weight of SAA included in breast milk was 12 kDa the same as maternal blood, but CRP had a difference among the two materials in contrast with SAA. As for the molecular weight of CRP included in colostrum, different two molecules were observed. One was 23 kDa with a known molecule same as being included in the maternal blood, the other was larger in about 50 kDa. Though CRP had not been included in feces of infant after ablactation, the CRP molecules included in neonatal feces after lactation was molecular weight of 23 kDa the same as breast milk. Furthermore CRP was also contained in meconium, but the molecular weight was not identified. This is the initial report that showed that SAA included in human breast milk is arrived in maximum on the third puerperium day, and showed that CRP have different molecular weight between maternal blood and colostrum whereas SAA don't have a difference. That SAA in mammary gland epithelial cells is induced by prolactin or lipopolysaccharide, that SAA increases intestinal mucin to provide a protective barrier and inhibits adhesion to the gut wall of enteropathogenic Escherichia coli has been recently reported by other researchers. When we consider them and evaluate this study, it is speculated that SAA and CRP which would be transferred from mother to a newborn infant through colostrum carry an important role to confer phylaxis ability on the newborn infant.Human whey serum amyloid A proteinC-reactive proteinColostrumWestern blottingUmbilical cord blood
東北大学の論文
2004-07-31

周産期における各種体液中のSAAならびにCRPの定量・定性解析と免疫生物学的意義

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